Kenney Laboratory

Dr. Scott Kenney
Food Animal Health Research Program

168 Food Animal Health/207 Hayden
OARDC Wooster, OH 44691
Phone: (330) 263-3747
Fax:(330) 263-3677
Email: kenney.157@osu.edu

Areas of Expertise

Animal Health, Veterinary Epidemiology, Infectious Disease Epidemiology, Veterinary Diagnostics, Veterinary Preventive Medicine, Veterinary Microbiology, Veterinary Infectious Diseases, Veterinary Virology, Veterinary Vaccine Production

Research Interests

Hepatitis E Virus (HEV) is an emerging zoonotic disease afflicting developing countries in Africa, Asia and the Americas where large outbreaks occur due to lack of sanitary drinking water. In developed countries in Europe and the Americas HEV is a zoonotic pathogen shown to pass primarily from pigs and pork products to humans. While generally healthy adults can fight off HEV, in pregnant women there is up to a 30% mortality rate and HEV can cause chronic hepatitis in immunocompromised individuals. There are currently 7 known genotypes within the Orthohepevirus A species (of human health concern). Genotype-1 infects humans and is found in Asia and Africa, genotype 2 also specifically infects humans and is more common in Mexico. Genotypes 3 and 4 are known to infect humans as well as swine, deer, rabbits, and mongoose. Genotypes 5, 6, and 7 infect animals including wild boar and camel. Orthohepevirus B-D infect chickens, bats, and ferrets, respectively, but are not known to infect humans.
Historically HEV has been difficult to propagate in cell culture and animal models were limited to pigs and chickens, limiting much of our understanding of the molecular aspects of HEV infection. Part of our ongoing research focuses on understanding cross species transmission of the virus, the factors required for efficient virus growth, and the creation of better small animal models to study this virus. Using this knowledge we hope to discover more about how HEV interacts with its hosts and create therapies to treat it.

Selected Publications

Li W*, Hulswit RJG*, Kenney SP*, Widjaja I, Jung K, Alhamo MA, van Dieren B, van Kuppefeld FJM, Saif LJ, Bosch BJ. Broad receptor engagement of an emerging global coronavirus may potentiate its diverse cross-species transmissibility.  Proc Natl Acad Sci U S A. 2018 May 14. [Epub ahead of print] PMID:29760102 DOI: 10.1073/pnas.1802879115

Kenney SP, Wentworth JL, Heffron CL, Meng XJ. Replacement of the hepatitis E virus ORF3 protein PxxP motif with heterologous late domain motifs affects virus release via interaction with TSG101. Virology. 2015 Dec;486:198-208. doi: 10.1016/j.virol.2015.09.012. Epub 2015 Oct 27. PMID:26457367

Kenney SP, Meng XJ. Therapeutic targets for the treatment of hepatitis E virus infection. Expert Opin Ther Targets. 2015;19(9):1245-60. doi: 10.1517/14728222.2015.1056155. Epub 2015 Jun 13. Review.PMID:26073772

Kenney SP, Meng XJ. An SH3 binding motif within the nucleocapsid protein of porcine reproductive and respiratory syndrome virus interacts with the host cellular signaling proteins STAMI, TXK, Fyn, Hck, and cortactin.  Virus Res. 2015 Jun 2;204:31-9. doi: 10.1016/j.virusres.2015.04.004. Epub 2015 Apr 13. PMID:25882913

Kenney SP, Meng XJ. Identification and fine mapping of nuclear and nucleolar localization signals within the human ribosomal protein S17. PLoS One. 2015 Apr 8;10(4):e0124396. doi: 10.1371/journal.pone.0124396. eCollection 2015. PMID:25853866

Kenney SP, Meng XJ. The lysine residues within the human ribosomal protein S17 sequence naturally inserted into the viral nonstructural protein of a unique strain of hepatitis E virus are important for enhanced virus replication. J Virol. 2015 Apr;89(7):3793-803. doi: 10.1128/JVI.03582-14. Epub 2015 Jan 21. PMID:25609799

Kenney SP, Pudupakam RS, Huang YW, Pierson FW, LeRoith T, Meng XJ. The PSAP motif within the ORF3 protein of an avian strain of the hepatitis E virus is not critical for viral infectivity in vivo but plays a role in virus release. J Virol. 2012 May;86(10):5637-46. doi: 10.1128/JVI.06711-11. Epub 2012 Mar 21. PMID:22438540

Cao D, Cao QM, Subramaniam S, Yugo DM, Heffron CL, Rogers AJ, Kenney SP, Tian D, Matzinger SR, Overend C, Catanzaro N, LeRoith T, Wang H, Piñeyro P, Lindstrom N, Clark-Deener S, Yuan L, Meng XJ.
Pig model mimicking chronic hepatitis E virus infection in immunocompromised patients to assess immune correlates during chronicity.
Proc Natl Acad Sci U S A. 2017 Jul 3;114(27):6914-6923. doi: 10.1073/pnas.1705446114. Epub 2017 Jun 19. PMID:28630341

Subramaniam S, Cao D, Tian D, Cao QM, Overend C, Yugo DM, Matzinger SR, Rogers AJ, Heffron CL, Catanzaro N, Kenney SP, Opriessnig T, Huang YW, Labarque G, Wu SQ, Meng XJ. Efficient priming of CD4 T cells by Langerin-expressing dendritic cells targeted with porcine epidemic diarrhea virus spike protein domains in pigs. Virus Res. 2017 Jan 2;227:212-219. doi: 10.1016/j.virusres.2016.10.007. Epub 2016 Oct 23. PMID:27784629

Modulation of Proinflammatory Cytokines in Monocyte-Derived Dendritic Cells by Porcine Reproductive and Respiratory Syndrome Virus Through Interaction with the Porcine Intercellular-Adhesion-Molecule-3-Grabbing Nonintegrin. Piñeyro PE, Subramaniam S, Kenney SP, Heffron CL, Giménez-Lirola LG, Meng XJ. Viral Immunol. 2016 Dec;29(10):546-556. Epub 2016 Sep 19. PMID:27643915

Carnaccini S, Shivaprasad HL, Cutler G, Bland M, Meng XJ, Kenney SP, Bickford AA, Cooper G, Charlton B, Sentíes-Cué CG. Characterization of Seven Outbreaks of Hemorrhagic Hepatopathy Syndrome in Commercial Pullets Following the Administration of a Salmonella Enteritidis Bacterin in California. Avian Dis. 2016 Mar;60(1):33-42. doi: 10.1637/11297-100915-Reg.1. PMID:26953941

Piñeyro PE, Kenney SP, Giménez-Lirola LG, Opriessnig T, Tian D, Heffron CL, Meng XJ. Evaluation of the use of non-pathogenic porcine circovirus type 1 as a vaccine delivery virus vector to express antigenic epitopes of porcine reproductive and respiratory syndrome virus. Virus Res. 2016 Feb 2;213:100-108. doi: 10.1016/j.virusres.2015.11.005. Epub 2015 Nov 7. PMID:26555162

Piñeyro PE, Kenney SP, Giménez-Lirola LG, Heffron CL, Matzinger SR, Opriessnig T, Meng XJ. Expression of antigenic epitopes of porcine reproductive and respiratory syndrome virus (PRRSV) in a modified live-attenuated porcine circovirus type 2 (PCV2) vaccine virus (PCV1-2a) as a potential bivalent vaccine against both PCV2 and PRRSV. Virus Res. 2015 Dec 2;210:154-64. doi: 10.1016/j.virusres.2015.07.027. Epub 2015 Jul 31. PMID:26239318

Subramaniam S, Piñeyro P, Tian D, Overend C, Yugo DM, Matzinger SR, Rogers AJ, Haac ME, Cao Q, Heffron CL, Catanzaro N, Kenney SP, Huang YW, Opriessnig T, Meng XJ. In vivo targeting of porcine reproductive and respiratory syndrome virus antigen through porcine DC-SIGN to dendritic cells elicits antigen-specific CD4T cell immunity in pigs. Vaccine. 2014 Nov 28;32(50):6768-75. doi: 10.1016/j.vaccine.2014.10.005. Epub 2014 Oct 18. PMID:25446829

Cossaboom CM, Huang YW, Yugo DM, Kenney SP, Piñeyro P, Matzinger SR, Heffron CL, Pierson FW, Meng XJ. RNA transcripts of full-length cDNA clones of rabbit hepatitis E virus are infectious in rabbits. J Gen Virol. 2014 Nov 7. pii: vir.0.071407-0. doi: 10.1099/vir.0.071407-0. Epub 2014 Nov 7. Retraction in: J Gen Virol. 2015 May;96(Pt 5):1190. PMID:25381057

Sanford BJ, Opriessnig T, Kenney SP, Dryman BA, Córdoba L, Meng XJ. Assessment of the cross-protective capability of recombinant capsid proteins derived from pig, rat, and avian hepatitis E viruses (HEV) against challenge with a genotype 3 HEV in pigs. Vaccine. 2012 Sep 28;30(44):6249-55. doi: 10.1016/j.vaccine.2012.08.013. Epub 2012 Aug 17. PMID:22906724

Cossaboom CM, Córdoba L, Sanford BJ, Piñeyro P, Kenney SP, Dryman BA, Wang Y, Meng XJ. Cross-species infection of pigs with a novel rabbit, but not rat, strain of hepatitis E virus isolated in the United States. J Gen Virol. 2012 Aug;93(Pt 8):1687-95. doi: 10.1099/vir.0.041509-0. Epub 2012 Apr 25. PMID:22535776

Pudupakam RS, Kenney SP, Córdoba L, Huang YW, Dryman BA, Leroith T, Pierson FW, Meng XJ. Mutational analysis of the hypervariable region of hepatitis e virus reveals its involvement in the efficiency of viral RNA replication. J Virol. 2011 Oct;85(19):10031-40. doi: 10.1128/JVI.00763-11. Epub 2011 Jul 20. PMID:21775444

Beach NM, Córdoba L, Kenney SP, Meng XJ. Productive infection of human hepatocellular carcinoma cells by porcine circovirus type 1. Vaccine. 2011 Oct 6;29(43):7303-6. doi: 10.1016/j.vaccine.2011.06.097. Epub 2011 Jul 8. PMID:21742002

Huang YW, Harrall KK, Dryman BA, Beach NM, Kenney SP, Opriessnig T, Vaughn EM, Roof MB, Meng XJ. Expression of the putative ORF1 capsid protein of Torque teno sus virus 2 (TTSuV2) and development of Western blot and ELISA serodiagnostic assays: correlation between TTSuV2 viral load and IgG antibody level in pigs. Virus Res. 2011 Jun;158(1-2):79-88. doi: 10.1016/j.virusres.2011.03.013. Epub 2011 Mar 23. PMID:21439334

Garbitt-Hirst R, Kenney SP, Parent LJ. Genetic evidence for a connection between Rous sarcoma virus gag nuclear trafficking and genomic RNA packaging. J Virol. 2009 Jul;83(13):6790-7. doi: 10.1128/JVI.00101-09. Epub 2009 Apr 15. PMID:19369339

Kenney SP, Lochmann TL, Schmid CL, Parent LJ. Intermolecular interactions between retroviral Gag proteins in the nucleus. J Virol. 2008 Jan;82(2):683-91. Epub 2007 Oct 31. PMID:17977961

Scheifele LZ, Kenney SP, Cairns TM, Craven RC, Parent LJ. Overlapping roles of the Rous sarcoma virus Gag p10 domain in nuclear export and virion core morphology. J Virol. 2007 Oct;81(19):10718-28. Epub 2007 Jul 18. PMID:17634229

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